Early diagnosis of American foulbrood by investigation of worker honey bees | Ранна диагностика на американския гнилец чрез изследване на пчелите работнички
2013
Gurgulova, K., National Diagnostic and Research Veterinary Institute, Sofia (Bulgaria) | Bonovska, M., National Diagnostic and Research Veterinary Institute, Sofia (Bulgaria) | Petrov, P., Agricultural University, Plovdiv (Bulgaria) | Takova, S., National Diagnostic and Research Veterinary Institute, Sofia (Bulgaria) | Petkov, N., National Breeding Beekeeping Association, Plovdiv (Bulgaria)
Levels of American foulbrood disease AFB were established from the investigations on a Project of National beekeeping programme for a development of beekeeping in Bulgaria in the Spring and Autumn of 2011-2012. A study of 320 bee samples of adult workers from 57 beekeeping farms was conducted in different regions of the country. Adult honey bee samples were pasteurized, centrifuged, plated on MYPGP agar and then the number of Paenibacillus larvae colonies was counted. P. larvae colonies were confirmed by Vita Europe Ltd. AFB test and 16S rRNA gene based PCR. Results indicate that Paenibacillus larvae spores were found only in 2 samples in autumn of 2012 - from the districts of Sofia and V. Tarnovo. Number of Paenibacillus larvae spores was about of 103 CFUs/plate of 0,2 mL suspension from 20 adult bees/10 mL distilled water. Number of CFUs from adult bee samples is a good predictor of AFB on a subclinical level. Results suppose that the regular monitoring of bee samples for the presence of Paenibacillus larvae spores and assessment of the epidemiological situation for the disease can reduce the risk of manifestation of American foulbrood before clinical symptoms are present.
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