Ultrastructural evaluation of canine layer intestinal muscle mantained in different conservative solutions | Análise ultra-estrutural da túnica muscular do intestino delgado de cães preservado em diferentes meios
2002
Francisco Cláudio Dantas Mota | Duvaldo Eurides | Marcelo Emílio Beletti | Patrícia Maria Coletto Freitas | Eneida César Mastrantonio | Bianca Jacob Shimizu | Julio Roquete Cardoso | Alan Kardek Martins
The present study evaluated twenty-five sarnples of the muscular layer of the small gut in dogs, which were preserved in a 300% sugar supersaturated. solution, 98% glycerin, 5% polyvinil-pirrolidona and tiomersal tincture at 1: 1000. The samples were maintained under conservation for 45 days and further submitted to ultrastructural assay by transmission electron microscopy. The cellular integrity was not entirely preserved when using the media, while the 300% sugar solution and 98% glycerin were the preservation conditions that better maintained the cellular ultrastructure.
Show more [+] Less [-]Foram avaliados 25 amostras da camada muscular do intestino delgado de cães, conservados em solução supersaturada de açúcar a 300%, glicerina a 98%, polivinil-pirrolidona a 5% e tintura de tiomersal 1:1000. As amostras foram mantidas conservadas por 45 dias e submetidas a análise ultra-estrutural por microscopia eletrônica de transmissão. Os meios não preservaram totalmente a integridade celular, sendo as soluções de açúcar a 300% e glicerina 98% as que melhor mantiveram as ultra-estruturas celulares.
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