Evolution analysis of the insect chemosensory protein gene family | 昆虫化学感受蛋白基因的进化分析
2011
Liu Xiaolei, South China Agricultural University, Guangzhou(China) | Weng Qunfang, South China Agricultural University, Guangzhou(China) | Hu Meiying, South China Agricultural University, Guangzhou(China)
Chinese. 摘 要：从基因库中调取已完成测序的昆虫化学感受蛋白（chemosensory proteins,CSPs）基因序列和氨基酸序列,共涉及昆虫7个目23个种的59个基因。应用MEGA 5.0形成了这59个基因的系统发育关系树,并计算CSPs基因在昆虫中的核苷酸替换速率。对非同义替换速率和同义替换速率进行比较的结果显示,CSPs基因在昆虫中总体上受到负选择作用。用PAML软件位点-特异模型检测到6个受到正选择作用的位点,其中5个位于功能重要的区域。
Show more [+] Less [-]English. The phylogenetic trees of the insect chemosensory proteins（CSPs） gene from 59 sequences of CSPs in 23 species under 7 orders published in GeneBank were generated by MEGA 5.0.Complete comparisons of CSPs sequences were used to determine the rates of synonymous（Ks） and nonsynonymous（Kn） codon substitutions and the ratio of Kn/Ks was calculated to estimate of the selection pressure on CSPs genes in insects.The Kn/Ks ratio was far less than 1,suggesting that CSPs may be evolving under negative selection.By the site-specific model in PAML 4.4,6 amino acid sites undergoing positive selection were detected,5 of them were in the important function regions.
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