Isolation, identification and anti-fungal action of endophytic fungi of rapeseed | 油菜内生真菌的分离鉴定及抑菌作用
2011
Lan Nan, Huazhong Agricultural University, Wuhan(China), College of Life Science and Technology | Qi Gaofu, Huazhong Agricultural University, Wuhan(China), College of Life Science and Technology | Yu Ziniu, Huazhong Agricultural University, Wuhan(China), College of Life Science and Technology
Chinese. 摘 要：从油菜幼苗中通过PDA培养基分离出9株内生真菌，通过形态观察和18SrRNA基因序列分析，其中6株鉴定为链格孢菌（Alternaria alternata），1株鉴定为球毛壳菌（Chaetomium globosum），1株属于镰刀菌属（Fusarium），1株属于炭角菌目（Xylariales）。从鉴定出的油菜内生真菌中，筛选出1株有抑菌活性的生防真菌――球毛壳菌（Chaetomium globosum）YY-11，此菌株对油菜菌核病菌（Sclerotinia sclerotiorum）、立枯丝核菌（Rhizoctonia solani）、油菜灰霉病菌（Botrytis cinerea）、小麦赤霉菌（Fusarium graminearum）等有不同程度的抑制作用。球毛壳菌YY-11对立枯丝核菌的玻片对峙镜检结果显示：在对立枯丝核菌的抑制过程中，球毛壳的拮抗机理主要是重寄生作用。
Show more [+] Less [-]English. Nine strains of endophytic fungi were isolated from rapeseed and classified into 4 genera through morphological and molecular biological identification. Plate confronting test showed that the endophytic Chaetomium globosum had different inhibition on Sclerotinia sclerotiorum , Rhizoctonia solani , Botrytis cinerea and Fusarium graminearurn , etc. The mechanism of antagonism C. globosum and R. solani with confrontation on slide found that C. globosum had apparent process of mycoparasitism during the confrontation with R. solani.
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