Effects of Aeromonas hydrophila injection on related immunity factors of red swamp crayfish Procambarus clarkii | 嗜水气单胞菌对克氏原螯虾免疫相关因子活性的影响

Chinese. 摘　　要：将嗜水气单胞菌（Aeromonas hydrophila）用灭菌生理盐水调节至1．5×10. cfu／mL（I组）和1．5×10. cfu／mL（Ⅱ组）的浓度后，注射法接种克氏原螯虾（Procambarus clarkii）（人工感染），定时检测供试克氏原螯虾的总血细胞数量（THCs）、淋巴液中酚氧化酶（PO）、超氧化物歧化酶（SOD）、溶菌酶活性和抗茵活力等免疫相关因子活性的变化状况。结果表明，注射不同浓度的A．hydrophila后的8～48h之间，供试克氏原螯虾的免疫相关因子的活性发生了显著变化。2个试验组的克氏原螯虾在注射A．hydrophila后8～48h内，THCs和SOD活性显著低于注射生理盐水的对照组，其中I组克氏原螯虾的THCs和SOD活性分别于注射感染的12、24h降至最低值，于48、96h恢复至正常值；而Ⅱ组克氏原螯虾的THCs和SOD活性均于48h降至最低值，于96h逐渐恢复。I组克氏原螯虾在注射A．hydrophila后12-48h，溶菌活力、抗菌活力和PO活性均显著高于对照组，并分别于24、24、48h达到最大值，96h后恢复正常；而Ⅱ组克氏原螯虾在注射A．hydrophila后12-48h，溶菌活力、抗菌活力和PO活性均显著低于对照组，均于48h降至最低，96h后逐渐恢复接近正常值，但是仍然低于对照组。

English. Healthy red swamp crayfish, Procambarus clarkii were artificially infected by injected with Aeromonas hydrophila at the concentration of 1.5× 10. （Group I ） and 1.5×10. cfu/mL（Group Ⅱ ）. Immunity factors including total haemocyte counts （THCs）,phenoloxidase （PO） activity, superoxide dismutase （SOD）, lysozyme activity and antibacterial activity in the haemolymph were examined at 1-96 h post infection at regular time. The results showed significant differences were observed in immune indicators of two infected groups during 8-48 h post infection. THCs and SOD activities of two treatment groups significantly decreased after 8-48 h compared to control, and THCs, SOD activities of group I decreased to the lowest level after 12,24 h,and returned to original level after 48,96 h respectively. However, THCs, SOD activities of group Ⅱdecreased to the lowest level after 48 h, and recovered gradually after 96 h. Lysozyme activity, antibacterial activity and PO activity of group I were significantly higher than those of the control group after 12-48 h,and reached to the top level after 24,24 and 48 h, respectively,and finally returned to original level after 96 h. Significant decrease was observed in lysozyme activity,antibacterial activity and PO activity of group Ⅱ after 12-48 h, and the lowest level was observed after 48 h, then gradually recovered after 96 h.