Polymorphisms of OSTF1 gene and their relationship with somatic cell score in Holstein cows | 荷斯坦母牛OSTF1基因多态性及其与体细胞评分的关系

Chinese. 摘　　要：设计5对引物P1-P5,利用PCR-SSCP技术检测227头荷斯坦母牛促破骨细胞因子1（osteoclaststimulating factor 1,OSTF1）基因部分序列的多态性。结果发现只有P3扩增的外显子7区域存在A45416G和C45436T2个突变位点,其中A45416G突变位点导致蛋氨酸（M）变为缬氨酸（V）。经SSCP分析存在AA、AB、BB、AC和CC 5种基因型,其频率分别为0.515、0.366、0.075、0.035、0.009。用最小二乘法分析OSTF1基因多态性与荷斯坦母牛体细胞评分（somatic cell score,SCS）的关系,结果表明：对于P3扩增产物多态位点,BB基因型SCS最小二乘均值显著低于AA、AB、AC基因型所对应的值（均为P〈0.05）;AA、AB、AC之间SCS最小二乘均值差异不显著（P〉0.05）。BB基因型为乳房炎抗性基因型。在中国荷斯坦奶牛群体中,OSTF1基因BB基因型可作为改良奶牛乳房炎抗性性状的分子遗传标记。

English. The genetic polymorphisms of partial sequence of OSTF1（osteoclast stimulating factor 1）gene in 227 Holstein cows were detected by PCR-SSCP with five pairs of primers P1-P5.Two polymorphic sites,A45416G and C45436T of primer P3 were found,and A45416G mutation caused amino acid change（methionine→valine）.Five genotypes（AA,AB,BB,AC and CC） were found in Holstein cows,and the genotype frequencies of AA,AB,BB,AC and CC were 0.515,0.366,0.075,0.035 and 0.009,respectively.The relationship between polymorphisms of OSTF1 gene and somatic cell score（SCS） in Holstein cows was analyzed by least squares linear model.Least squares mean of SCS for BB genotype was significantly lower than that for AA,AB or AC（P〈0.05）,and no significant difference was found in least squares mean of SCS between other genotypes（P〉0.05）.So genotype BB was mastitis resistant and it may be used as a molecular genetic marker to improve the phenotype of anti-mastitis in Chinese Holstein dairy cows.