Research on SSR molecular marker identification technique of hybrids purity in cabbage | 甘蓝一代杂种纯度SSR鉴定与田间鉴定的一致性研究

Chinese. 分析甘蓝杂交种纯度的SSR分子标记鉴定结果与田间形态学鉴定结果的一致性，寻找一种快速、准确鉴定甘蓝杂交种纯度的方法。选用SSR分子标记，筛选杂交种具有父母本互补带型的引物，用其鉴定“秦甘50”杂交种的纯度，并与田间常规鉴定结果的一致性进行比较。300对SSR分子标记引物中，有1对引物SQ1019对“秦甘50”杂交种表现为父母本互补带型；利用引物SQ1019标记鉴定“秦甘50”大田杂交种的纯度为96.3%；“秦甘50”大田杂交种经田间鉴定纯度为96.0%，2种鉴定结果的一致性高达99.7%。SQ1019是SSR分子标记“秦甘50”杂交种的特异引物，SSR分子标记是一种快速、准确鉴定“秦甘50”杂交种纯度的方法，其结果与田间常规鉴定结果相一致。

English. The research analyzed consistency of SSR molecular marker technique with field morphological observation of hybrids purity in cabbage to seek a quick and accurate identification of seed purity. We screened out stable codominance banding pattern SSR primers by using SSR molecular marker technique, identified the purity of Qin’gan 50 hybrids and compared with the result of morphological observation in field. There were 1 pair of primers SQ1019 which presented stable codominance banding pattern among 300 pairs of screened SSR primers; The purity of Qin’gan 50 was 96.3% by SSR molecular marker using SQ1019; Field identification with its corresponding plant of SSR was 96.0%. The results indicated that they were the same for hybrid identification between SSR approach and morphological assessment, with the consistency rate of them reaching 99.7%. SQ1019 is the specific primer to identify seed purity of Qin’gan 50, the identification between SSR approach and morphological assessment is the same, SSR molecular marker technique is a quick and accurate method to identify seed purity.