Effects of dietary supplemental compound of Chinese herbal medicine on caecum inner circumstance and mRNA expression of IFN-γ and IL-10 in spleen in weaned rex rabbits | 复方中药对断奶獭兔盲肠内环境及脾脏IFN-γ、IL-10 mRNA表达的影响

Chinese. 研究复方中药（cCHM）添加剂对断奶獭兔盲肠内环境及脾脏IFN-γ、IL-10 mRNA表达的影响，探讨其对机体抗病能力的调控作用及适宜用量。选用（35±2）日龄断奶、体质量相近的獭兔144只，随机分为对照组和3个试验组，每组6个重复，每重复6只幼兔。对照组饲喂添加0.1 g/kg杆菌肽锌的日粮（不含抗生素等其他药物），试验组分别饲喂添加复方中药5 g/kg（cCHM1组）、10 g/kg（cCHM2组）、20 g/kg（cCHM3组）的日粮，60 d后，取脾脏和盲肠样品，测定盲肠微生物活力和数量，检测IFN-γ、IL-10的相对表达量。与对照组相比，cCHM2组獭兔盲肠内容物氨氮（NH3-N）浓度降低21.62%，菌体蛋白（MCP）含量提高16.81%，差异显著（P＜0.05）。复方中药抑制大肠杆菌的效果不及抗生素，但cCHM2组、cCHM3组乳酸杆菌数量较对照组分别提高18.31%和16.50%，差异显著（P＜0.05）。复方中药提高了脾脏IFN-γ、IL-10 mRNA的相对表达量，cCHM2组和cCHM3组均极显著高于对照组（P＜0.01）。复方中药添加剂可改善断奶獭兔盲肠内环境，增强机体的免疫功能，有利于断奶獭兔的肠道健康；该试验条件下复方中药添加剂的适宜用量为10 g/kg。

English. The trial was designed to study the effects of dietary supplemental compound of Chinese herbal medicine (cCHM) on caecum inner circumstance and mRNA expression of IFN-γ and IL-10 in spleen in weaned rex rabbits. The regulatory effect of cCHM on disease resistance of weaned rex rabbits was probed, and the appropriate supplemental amount was investigated. A total of one hundred and forty-four weaned rex rabbits with (35±2) days of age and similar body weight were randomly divided into the control group and 3 experimental groups, with 6 replicates in each group and 6 rabbits per replicate. The control group was fed diet supplemented with 0.1 g/kg zinc bacitracin, and the experimental groups with 5 g/kg (cCHM1 group), 10 g/kg (cCHM2 group) and 20 g/kg (cCHM3 group) cCHM, respectively. After 60 days, caecum and spleen were collected to measure the caecum microorganism vitality and quantity, and to detect mRNA relative expression of IFN-γ and IL-10 in spleen. Compared with the control group, cCHM2 group significantly decreased ammonia nitrogen (NH3-N) concentration by 21.62% and increased microbial protein (MCP) by 16.81% (P＜0.05). Although the result of inhibiting E. coli in the experimental groups was not better than the control group, both cCHM2 group and cCHM3 group increased the amount of Lactobacilli by 18.31% and 16.50% (P＜0.05), respectively. cCHM improved mRNA relative expression in spleen, compared with the control group, both cCHM2 group and cCHM3 group increased mRNA relative expression of IFN-γ and IL-10 (P＜0.01). cCHM as dietary additive can improve weaned rex rabbits caecum inner circumstance and immune function, which is beneficial to intestinal health. The appropriate supplemental amount of compound Chinese herbal additive is 10 g/kg under this experimental condition.