Studies on selecting high-yield poly-β-hydroxybutyrate producing strain by heavy-ion irradiation | 重离子诱变选育聚β-羟基丁酸酯高产菌株研究
2012
Zhang Nan, Lanzhou Jiaotong University, Lanzhou (China), College of Chemical and Biology Engineering | Zhang Binglin, Lanzhou Jiaotong University, Lanzhou (China), College of Chemical and Biology Engineering | Wang Wan’r, Lanzhou Jiaotong University, Lanzhou (China), College of Chemical and Biology Engineering
Chinese. 聚β-羟基丁酸酯(PHB)作为一种新型的生物可降解高分子材料，日益受到人们的广泛重视。利用12C6+离子束对一株聚β-羟基丁酸酯（PHB）产生菌――芽孢杆菌Z-3进行诱变，然后进行筛选，从大量突变株中最终选育出一株PHB高产菌株A11，其PHB产量达到1.404 g/L，是出发菌株的1.33倍。研究结果揭示，重离子诱变育种技术具有良好的诱变效果，是一种有效的微生物诱变育种方法和途径。
Show more [+] Less [-]English. As a new biodegradable polymer materials, Poly-β-hydroxybutyrate (PHB) has attracted wide attention from people day by day. This study adopts heavy-ion mutation breeding technology, using 12C6+ ion beams to mutate the PHB producing strain-Bacillus Z-3, then screen a PHB high yielding strain A11 from a mount of mutant strains. Its PHB output can reach 1.404 g/L. This is 1.33 times as compared with the original strain. The results indicate that heavy-ion mutation breeding technology has good mutagenic effects. It is an effective method and way to microbial mutation breeding.
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