Physiological and Biochemical Response of Albizzia kalkora Seeds to Ultra-dry Storage | 山合欢种子超干燥贮藏的生理生化效应

Chinese. 采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来探索山合欢种子超干燥保存的可行性。用硅胶将种子脱水至1.42%～6.35%6个含水量梯度,结合不同处理密封保存1年,随后测定发芽率和一些生理生化指标,包括相对电导率（RC）、脯氨酸（Pro）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、可溶性总糖含量和脂肪酸组分。结果表明：种子含水量和预回湿方法对发芽率有显著影响,超干种子与未经超干处理的种子相比,发芽率有明显提高;超干种子发芽率与相对电导率和丙二醛含量呈显著负相关,与脯氨酸含量和3种抗氧化酶活性呈显著正相关;超干种子的油酸和α-亚麻酸含量比对照提高。适宜的超干处理能使抗氧化酶活性增强,使不饱和脂肪酸和可溶性总糖的含量增加,从而减缓种子生活力下降。

English. An orthogonal design, including four factorial （seed moisture content, packing method, storage temperature and pre-humidification method） , used to study the feasibility of ultra-dry storage of Albizzia kalkora seeds. Seeds were dried to different moisture contents ranging from 1.42% to 6.35% with silica gel, and then stored in a hermetic condition combined with other treatments for a year. After storage the seed germination percentage, electrical conductivity （RC） , proline （Pro）, malondialdehyde （MDA）,＇superoxide dismutase （SOD）, peroxidase （POD）, catalase （CAT）, total soluble sugar content and fatty acid composition were tested. The results showed that the seed moisture content and pre- humidification method had a significant effect on germination percentage. Germination percentage of the uhra-dry seeds was increased compared to control seeds. The germination percentage was significantly negatively correlated with electrical conductivity and proline content, and was significantly positively correlated with proline content and activity of three antioxidant enzymes. The content of oleic acid and ct-Linolenic acid in the ultra-dry seeds were higher than the control seeds. Appropriate ultra-dried treatment could increase the activity of antioxidant enzymes and the content of unsaturated fatty acid and total soluble sugar, which would alleviate the seed deterioration.