Microtubule Organization in Pollen and Pollen Tube of Cunninghamia lanceolata | 杉木花粉和花粉管中的微管分布
2012
Gan Guofeng, Nanjing Forestry University，Nanjing (China) | Lu Ye, Nanjing Forestry University，Nanjing (China) | Xu Jin, Nanjing Forestry University，Nanjing (China)
Chinese. 通过间接免疫荧光标记对杉木花粉和花粉管内微管骨架的分布进行观察,结果显示：杉木花粉在萌发初期的花粉中存在浓密的微管网络,主要呈现横向或斜向分布;花粉萌发后,微管开始从花粉粒延伸入花粉管中,花粉粒和花粉管基部微管排列呈现横向、斜向或螺旋状排列;在杉木花粉管中部微管骨架则主要呈现与花粉管伸长相平行的网络状分布,在部分伸长初期的花粉管中微管会延伸到花粉管顶端。在多数花粉及花粉管中微管主要分布在细胞的周质。在花粉管顶端微管分布有3种形式：1）在花粉管亚顶端有垂直于花粉管伸长方向排列的微管;2）花粉管中微管呈螺旋状分布并延伸到顶端;3）微管在花粉管顶端形成复杂的网络结构。
Show more [+] Less [-]English. Microfilaments in the pollen and pollen tube of Cunninghamia lanceolata was observed by indirect immunofluorescence labeling and a confocal laser scanning microscope. We found that microtubules were arranged obliquely or transversely in the germinated pollen. After pollens germinated, microtubules in pollen grain and the basal of pollen tube formed an oblique or transverse array. In the middle of pollen tube, microtubules were arranged along the longitudinal axis. In some germinated pollen tubes, microtubules were arranged obliquely or transversely and extended to the pollen tube apex. Most microtubules were localized in the cortical of the pollen and pollen tube. In the pollen tube apex, there were different microtubule distributions as following： 1 ） Microtubules formed an array beneath the plasma membrane that was perpendicular to the direction of tube growth. 2） Microtubules were arranged obliquely or transversely in the pollen tube apex. 3） Microtubules formed a complex network in the tip of pollen tube.
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