Grey Correlation Analysis between Main Climatic Factors and Active Compounds, Antioxidant Activity of Potentilla glabra Leaves | 银露梅叶有效成分及抗氧化活性与气候因子的灰色关联分析

Chinese. 为明确影响银露梅叶有效成分及抗氧化活性的主导气候因子,揭示银露梅生长的适宜生态区和生境特征,以及为充分开发利用和合理保护野生银露梅药用资源提供依据。采用灰色关联度分析研究 6 个不同产地野生银露梅叶中单宁、黄酮含量及体外抗氧化活性与气候因子的相关性。在单因素试验基础上,采用响应曲面法优化银露梅叶单宁、黄酮的提取工艺。单宁含量测定采用钨酸钠 - 磷钼酸显色法,黄酮含量测定采用亚硝酸钠 - 硝酸铝比色法,抗氧化活性测定采用体外 DPPH・自由基清除法。结果表明： 不同产地银露梅品质存在显著差异,单宁含量为 4. 95% ～ 7. 74% ; 黄酮含量为 1. 07% ～ 3. 56% ,其中青海互助产银露梅叶中的单宁、黄酮含量均最高,分别达7. 74% 和 3. 56% ; 贺兰山产银露梅叶的抗氧化能力最强,其 DPPHIC50值为 6. 9 μg・mL- 1; 灰色关联度分析显示影响单宁含量的主导因子是年极端最低温度、无霜期和年极端最高温度; 影响黄酮含量的主导因子是年日照、年极端最低温度和年积温; 影响抗氧化活性的主导因子是年日照、年均降雨量和年极端最低温度。

English. To reveal suitable ecological zones and habitat characteristics about the growth of PotentiUa glabra and to provide evidences for developing fully and protecting reasonably wild medical P. glabra resource we investigated and determined the major climatic factors that affect P. glabra production. P. glabra leaves collected from different sample plots were used for measuring the tannin, total flavonoids content and antioxidant activity. The response surface method was applied to optimize the extraction process of tannin and total flavonoids from P. glabra leaves with one-factor experimental design. The tannin content was determined with sodium tungstate-phosphomolybdic acid approach, the content of total flavonoids was determined with NaNO2-Al（ NO3 ） 3 approach, and the antioxidant activity was determined with the DPPH ・ method. The relationship between variation in the parameters in six different sampling sites and the corresponding major climatic factors was analyzed with gray correlation degree analysis. The result showed that the quality of P. glabra was significantly different in terms of the parameters among the six different regions. Tannin content ranged from 4.95% to 7.74% , total flavonoids content ranged from 1.07% to 3.56%. P. glabra from Huzhu region had the highest tannins and flavonoids product, respectively 7.74% and 3.56% , while P. glabra in Helan mountains had the strongest antioxidant activity and the value of DPPHIIC50 was 6.9 μg. mL 1 ; Grey correlation degrees analysis indicated that the annual extreme minimum temperature, frost fi＇ee periods and the annual extreme maximum temperature were the main climatic factors affecting tannin content; Annual sunshine, the annual extreme minimum temperature and annual accumulated temperature were the major climatic factors influencing total flavonoids content, and annual sunshine, annual mean rainfall and the annual extreme minimum temperature were the major factors for regulating antioxidant activity.