Effects of calcium on soluble protein expression of cucumber seedlings under root-zoon hypoxia stress | 钙对根际低氧胁迫下黄瓜幼苗可溶性蛋白表达的影响

Chinese. 采用营养液水培,以低氧耐性较弱的黄瓜品种‘津春2号’为试材,研究了外源钙和钙调素（Ca.M）抑制剂三氟拉嗪（TFP）对根际低氧胁迫下黄瓜幼苗根系和叶片可溶性蛋白表达的影响。结果表明：低氧下植株的生物量显著下降,施加外源Ca2＋后生物量显著增加,而施加TFP后则在低氧胁迫的基础上进一步降低了植株的生物量。植株的根系可溶性蛋白含量在低氧下降低而在添加了Ca2＋后升高;叶片的可溶性蛋白含量在低氧下升高,添加Ca2＋后可溶性蛋白在处理6 d和9 d时高于低氧和对照处理;施加TFP后根系和叶片的可溶性蛋白均下降。SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析表明,不同处理在根系中至少有9种蛋白表达量发生明显变化,其相对分子质量（×103）分别为17.2、19.7、24.0、31.5、35.0、51.2、64.8、75.0、83.0;叶片中也有7种蛋白表达量发生了变化,其相对分子质量（×103）分别为14.0、21.0、34.0、37.2、39.0、46.5、58.5。外源Ca2＋显著增加了黄瓜的生物量,调节根系和叶片中可溶性蛋白含量及其表达,缓解了低氧胁迫对植株的伤害,增强了黄瓜的低氧耐性。

English. A hydroponic experiment was conducted to study the effects of exogenous calcium and trifluoperazine（TFP）on the soluble protein expression of hypoxia sensitive cultivar of’Jinchun 2’cucumber（Cucumis sativus L.）under root-zoom hypoxia stress.The results showed that the biomass of plants significantly decreased under hypoxia.After exogenous Ca2＋ was added in the nutrient solution,the biomass of plants increased significantly,but decreased sharply after adding in the TFP compared with hypoxia treatment.The soluble protein content of root decreased under hypoxia and increased after exerting Ca2＋.The soluble protein of leaves increased under hypoxia,and under Ca2＋ treatment was higher than the control and hypoxia treatment at the sixth and the ninth day after treatment;the soluble protein of root and leaves decreased both under TFP treatment.Through the SDS-PAGE analysis,9 proteins relative molecular weight（×103）of 17.2,19.7,24.0,31.5,35.0,51.2,64.8,75.0 and 83.0 changed obviously in roots under different treatments;7 proteins relative molecular weight（×103）of 14.0,21.0,34.0,37.2,39.0,46.5 and 58.5 also changed in leaves.The exogenous calcium increased the biomass significantly,and adjusted the soluble protein content and protein expression in roots and leaves of cucumber,and then enhanced the hypoxia tolerance of cucumber.