Effect of oral immunization with inactivated avian influenza virus and adjuvant on the antibody secreting cells in duck digestive tract | 禽流感灭活抗原与佐剂配合饮水免疫对鸭消化道抗体分泌细胞的影响

Chinese. 为探讨鸭源禽流感灭活抗原与佐剂配合饮水免疫雏鸭的效果,应用鸭源禽流感灭活抗原与复合黏膜免疫佐剂（CpG和/或葡萄糖）饮水免疫雏鸭,检测消化道（主要是咽和小肠）抗体分泌细胞的变化。首先分别从鸭的胆汁和血清中粗提免疫球蛋白A（IgA）和IgG,经纯化后制备了兔抗鸭IgA和IgG,然后应用免疫组化技术显示鸭消化道IgA和IgG分泌细胞。试验结果表明：用禽流感灭活抗原配合CpG和/或葡萄糖饮水免疫后3、5和7周,消化道黏膜局部IgA分泌细胞面积均显著或极显著增加（P〈0.05或P〈0.01）,IgG分泌细胞（除首免后第3和7周小肠外）极显著升高（P〈0.01）。而单独用禽流感灭活抗原和用葡萄糖配合禽流感灭活抗原饮水免疫对消化道局部免疫水平影响不大。结论：鸭源禽流感灭活抗原配合免疫佐剂饮水免疫能够提高雏鸭消化道局部免疫水平。

English. In order to evaluate the effects of oral immunization with the inactivated avian influenza virus（iAIV）vaccine with adjuvant on ducklings.The area of IgA and IgG secreting cells in the digestive tract（mainly pharynx and small intestine）were detected after oral immunization with iAIV and adjuvant CpG with or without glucose.First,immunoglobulin A（IgA）and IgG was isolated from duck bile and serum respectively,and rabbit antiserum was isolated from inoculated rabbits by purified IgA and IgG.The antibody secreting cells were showed by SPA-HRP immunohistochemical technique.The results showed that the area of IgA and IgG secreting cells（except for the 3rd and 7th week in small intestine）increased significantly（P〈0.05 or P〈0.01）at the weeks 3rd,5th and 7th after oral immunization with iAIV and adjuvant CpG with/without glucose.No significant changes were found in the ducks immunized with iAIV only or iAIV and glucose.Conclusion：the oral immunization with iAIV together with adjuvants could elict local immune response in the digestive tract.Therefore,we speculated it appears to be efficacious in suppressing virus replication and shedding.