Effects of high producing methionine soybean transferred cystathionine γ-synthase gene on community structure of bacteria in soil | 转胱硫醚-γ-合酶基因高蛋氨酸大豆对土壤细菌群落结构的影响
2012
Pu Chuanliang, Nanjing Agricultural University,Nanjing (China),College of Life Sciences | Liang Jingang, Nanjing Agricultural University,Nanjing (China),College of Plant Protection | Gao Jinyu, Nanjing Agricultural University,Nanjing (China),College of Life Sciences
Chinese. 采用16S rDNA V3区PCR-DGGE技术,对转胱硫醚-γ-合酶基因高蛋氨酸大豆根际土壤细菌群落结构进行研究。结果表明：与非转基因大豆相比,转胱硫醚-γ-合酶基因高蛋氨酸大豆对土壤中部分酸杆菌门（Acidobacteria）和芽单胞菌门（Gemmatimonadetes）的细菌有一定的抑制作用,对部分变形杆菌门（Proteobacteria）和蓝菌门（Cyanobacteria）的细菌有一定的促进作用,从而改变了土壤细菌群落结构;时间变化是影响土壤细菌群落结构的主要因素,细菌群落多样性指数分析显示,转胱硫醚-γ-合酶基因高蛋氨酸大豆对细菌群落多样性有一定的降低作用,但这一作用随大豆生长时间的延长而逐渐减弱,到成熟期已完全消失。
Show more [+] Less [-]English. PCR-DGGE of 16S rDNA V3 region was used to research the effects of high producing methionine soybean transferred cystathionine γ-synthase gene on community structure of bacteria in soil.The results demonstrated that,compared with non-genetically modified soybean receptor,high producing methionine soybean transferred cystathionine γ-synthase gene inhibited the growth of some bacteria belonging to phylum Acidobacteria,Gemmatimonadetes,and stimulated the growth of others bacteria belonging to phylum Proteobacteria,Cyanobacteria.Thus,it changed the community structure of bacteria in soil.Time was the main factor which affected the community structure of soil bacteria,and the study of bacterial diversity in soil revealed that bacterial diversity was lowered in some degree.But the influence was weakened gradually with the growth of soybean,and disappeared when the soybean matured.
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