Effects of five phenolic allelochemicals on growth and enzymes activity of Chuzhou chrysanthemum cutting seedling | 5种酚酸物质对滁菊扦插幼苗生长及酶活性的影响

Chinese. 以滁菊扦插幼苗为材料,采用营养液水培方式研究对羟基苯甲酸、肉桂酸、阿魏酸、香草醛和水杨酸5种酚酸物质对其生理生化指标的影响。结果表明：5种酚酸物质在100μmol・L-1的高浓度处理中对滁菊幼苗的根长度、可溶性蛋白、可溶性糖、叶绿素a和叶绿素b含量产生显著抑制作用。在10和50μmol・L-1浓度处理下,过氧化物酶（POD）活性受到抑制,且增加了过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性,而在100μmol・L-1处理浓度时,3种酶的活性显著降低。随着酚酸物质浓度的增加,丙二醛（MDA）在根系中含量逐渐增加。在10和50μmol・L-1处理中,苯丙氨酸解氨酶（PAL）活性均高于对照,而在100μmol・L-1处理条件下,5种酚酸物质对PAL均呈现抑制作用,其酶活性均低于对照。5种酚酸物质浓度在100μmol・L-1时可以抑制根长度,减少滁菊叶绿素、可溶性蛋白、可溶性糖的含量,降低滁菊植株体内POD、CAT、SOD、PAL活性,增加MDA含量,从而对滁菊生长产生毒害作用。

English. An experiment with nutrient solution hydroponics was conducted to study effects of p-hydroxybenzoic acid,cinnamic acid,ferulic acid,vanillin and salicylic acid on growth and enzymes activity of cutting seedlings of Chuzhou chrysanthemum.The results showed that five phenolic allelochemicals at 100 μmol・L-1 concentrations had significant inhibitory effects on root length,and the contents of soluble sugar,soluble protein,chlorophyll a and chlorophyll b in root tissue.The results indicated that POD activity was inhibited,and CAT and SOD activity increased at 10 and 50 μmol・L-1 concentrations of five phenolic allelochemicals,and then decreased at 100 μmol・L-1 concentration,significantly.The content of MDA increased with higher concentrations of phenolic allelochemicals.Compared with the control without phenolic acids,the phenylanlanine ammonialyase（PAL）activity increased at 10 and 50 μmol・L-1,and by contrast,it was inhibited by five phenolic allelochemicals at 100 μmol・L-1.It proved that the poisoning effect of the five phenolic allelochemicals on Chuzhou chrysanthemum growth was caused by the way of inhibiting root length,and it decreased POD,CAT,SOD and PAL activities,and the content of chlorophyll a,chlorophyll b,soluble sugar and soluble protein,whereas increased the content of MDA in root tissue under high concentration.