Sequences analysis of CP genes in two isolates of Grapevine leafroll-associated virus1 from Hebei and Liaoning Province | 葡萄卷叶伴随病毒1号河北和辽宁分离物CP基因序列分析

Chinese. 为获得葡萄卷叶伴随病毒1号（Grapevine leafroll-associated virus 1,GLRaV-1）中国分离物的分子信息,提高其检测可靠性,本研究从河北怀来和辽宁兴城地区带有明显卷叶症状的葡萄样品中提取的叶柄韧皮部总RNA,采用RT-PCR克隆了GLRaV-1的河北（GLRaV-1-HB）和辽宁分离物（GLRaV-1-LN）的外壳蛋白（coatprotein,CP）基因,并进行了序列分析。结果表明：GLRaV-1-HB和GLRaV-1-LN的CP基因核苷酸同源性为90.7％,由此推导的氨基酸同源性为93.8％;与已报道的国外3个GLRaV-1分离物的CP基因全序列相比,其核苷酸与氨基酸序列同源性分别为90.0％～96.0％和92.9％-97.5％。以CP基因序列构建的进化树表明,现有的5个分离物可分为3组,其中本研究得到的GLRaV-1-HB和GLRaV-1-LN,分别与巴西分离物（GLRaV-1-PS,GenBank登录号为GQ332536.1）和伊朗分离物（GLRaV-1-IR-S7,FJ952151.2）为一组;澳大利亚分离物（GLRaV-1-AUS,AF195822.1）单独为一组。

English. In order to improve the detection reliability,grapevine leaves with typical leafroll symptom originated from Huailai region in Hebei and Xingcheng region in Liaoning Province were detected for GLRaV-1 by RT-PCR.The complete sequences of coat protein（CP） gene of GLRaV-1-HB and GLRaV-1-LN isolates were amplified.Sequence comparisons revealed that GLRaV-1-HB and GLRaV-1-LN shared 90.7% and 93.8% identities at nucleotide and amino acid levels,respectively.In contrast with the reported 3 foreign isolates,the identities of nucleotide sequence and deduced amino acid sequence ranged from 90.0% to 96.0% and 92.9% to 97.5%,respectively.The phylogenetic tree from alignments of CP nucleotide sequences of 5 GLRaV-1 isolates revealed the existence of three well-defined clusters.GLRaV-1-HB showed a close relationship with Isolate PS from Brazil（GLRaV-1-PS,GenBank accession number GQ332536.1）,while GLRaV-1-LN and Isolate IR-S7 from Iran（GLRaV-1-IR-S7,FJ952151.2）clustered together.An Australia Isolate（GLRaV-1-AUS,AF195822.1）was grouped into a cluster alone.