Optimization of pretreatment for determination of soluble sugar in straw | 秸秆可溶性糖测定时样品前处理条件的优化
2012
Niu Wenjuan, China Agricultural University,Beijing(China),College of Engineering | Xiao Weihua, China Agricultural University,Beijing(China),College of Engineering | Liu Xian, China Agricultural University,Beijing(China),College of Engineering
Chinese. 为确定秸秆可溶性糖测定时样品的最佳前处理条件,研究单因素对玉米秸秆和小麦秸秆可溶性糖得率的影响。利用中心组合试验（Box-Behnken）和响应曲面法（RSM）对主要前处理参数进行优化,并得到回归模型。结果表明：固液比（秸秆质量与水体积比）、水浴温度、提取时间和存放时间对玉米秸秆和小麦秸秆可溶性糖得率影响显著（P〈0.05）。回归方程较好的反映了可溶性糖得率与固液比、水浴温度和提取时间的关系。玉米秸秆可溶性糖测定的最佳前处理条件为,固液比0.31g/mL,水浴温度94℃,提取时间63min,此条件下的可溶性糖得率为3.85%。小麦秸秆可溶性糖测定的最佳前处理条件为,固液比0.33g/mL,水浴温度95℃,提取时间58min,此条件下的可溶性糖得率为3.39%。
Show more [+] Less [-]English. In order to determine the optimal pretreatment condition of soluble sugar in corn stover and wheat straw,different factors were studied,Box-Behnken and RSM were used for the optimization of pretreatment and regression models were obtained.The results showed that the ratio of straw to water,water-bath temperature,extraction time and stored time had significant effects,P〈0.05,on the yield of soluble sugar in corn stover and wheat straw.The optimal pretreatment condition of soluble sugar in corn stover is that the ratio of straw to water is 0.31 g/mL,water-bath temperature is 94 ℃,extraction time is 63 min,then the yield of soluble sugar in corn stover reach 3.85%.the optimal pretreatment condition of soluble sugar in wheat straw is that the ratio of straw to water is 0.33 g/mL,water-bath temperature is 95 ℃,extraction time is 58 min,then the yield of soluble sugar of wheat straw reach 3.39%.
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