Genetic diversity of Xanthomonas oryzae pv. oryzae in rice fields of Guilan province (Iran) using RAPD markers
2012
Shahrestani, A.T., University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Pathology | Kazempour, M.N., University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Pathology | Ebadie, A.A., Rice Research Inst. of Iran, Rasht (Iran Islamic Republic) | Elahinia, S.A., University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Pathology
Genetic variation in 60 isolates of Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of rice bacterial blight disease, was investigated using random amplified polymorphic DNA (RAPD) markers. The sixty strains isolated and characterized in Guilan province, Iran, during the years 2006-2008 were divided into three groups as follows: a) isolates from nursery, b) isolates from field, and c) isolates from panicle. Eight decamer primers of RAPD amplified 187 polymorphic bands. The largest and smallest fragments were generated by 80.7 and OPA10 primers, respectively. The minimum amount of polymorphic information content was related to OPA12 primer (0.36), the maximum one to OPA10 primer (0.44). Analysis of molecular variance of data from RAPD analysis showed that the genetic variation within Xoo populations was greater than between populations. The similarity matrix was conducted by Simple Matching coefficient and cluster analysis was made by unweighted pair group method with NTSYS-pc software. At a similarity index of 0.6 the isolates were grouped into 3 clusters. These results revealed that the RAPD markers used in this study could differentiate nursery and field isolates from each other.
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