The influence of high selenium intake of ewes on leukocytes in newborn lambs
2012
Pisek, L., Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta | Travnicek, J., Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta | Peksa, Z., Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta | Dusova, H., Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta | Konecny, R., Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta | Soch, M., Jihoceska Univ., Ceske Budejovice (Czech Republic). Zemedelska Fakulta
The aim of this work was to determine the impact of high selenium intake on pregnant ewes considering the number of leukocytes and the representation of T cell subsets in the blood of newborn lambs. The ewes (Sumava breed) were divided into three groups of five animals as follows: control C (without Se supplementation), experimental E1 (sodium selenite supplementation, Se concentration: 160 microg/kg DM) and E2 (Chlorella as a carrier for Se, Se concentration: 160 microg/kg DM). Blood samples were taken from 19 suckling newborn lambs on days 1, 3, 10, 30 and 60. The WBC counts and proportions of lymphocytes in blood smear were detected by microscopic analysis. The CD4+ and CD8+ T cell subsets in blood were detected by flow cytometry. The differences determined were not statistically significant due to high variabilities within the groups. In lambs whose mothers were supplemented with Se bound to Chlorella a statistically significant increase in the proportion of both subsets of T lymphocytes was proved. The results document a tendency to a lower level of the selected characteristics of immunity in lambs without Se supplementation.
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