Effect of cysteamine supplementation during in vitro culture of early stage bovine embryos on blastocyst rate and quality
2012
Lojkic, M., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Getz, I., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Samardzija, M., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Matkovic, M., Center for Reproduction and Animals Breeding of Croatia, Novi Zagreb (Croatia) | Bacic, G., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Karadjole, T., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Macesic, N., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Folnozic, I., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction | Spoljaric, B., University of Zagreb (Croatia). Clinic for Obstetrics and Reproduction
The aim of this study was to evaluate whether the addition of cysteamine to the in vitro culture media enhances the yield, hatching rate, total cell number and inner cell mass/total cell number ratio of bovine embryos. A total of 933 bovine oocytes collected from ovaries of 60 slaughtered donors were subjected to in vitro maturation and in vitro fertilization. Following fertilization, embryos were cultured in synthetic oviductal fluid without glucose. After 24 h embryos were transferred into synthetic oviductal fluid with 1.5 mM glucose and 0 (control), 50, 100 and 200 microM of cysteamine. After 48 h, the embryos were transferred into synthetic oviductal fluid with glucose but without cysteamine and cultured until Day 9. The number of cleaved embryos on Day 2, the total number of blastocysts on Day 7 and the number of hatched blastocysts on Day 9 were calculated. Differential staining of inner cell mass and trophectoderm cells of blastocysts were performed on Day 7 and Day 9 of in vitro culture. Supplementation of in vitro culture media with 100 microM cysteamine increased the blastocyst yield (P less than 0.05) without affecting the hatching rate. Furthermore, the embryos cultured in the presence of 100 microM cysteamine had significantly higher number of inner cell mass cells (P less than 0.05) and the proportion of inner cell mass cells (P less than 0.05) compared with the controls. The results of the present study demonstrated that the addition of 100 microM cysteamine to the in vitro culture media improved blastocyst production rate and enhanced embryo quality, which could lead to the improvement of the in vitro culture system for bovine embryos.
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