Intrafollicular LH administration in dairy heifers treated with a GnRH agonist
2013
Mala, J., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic) | Beckers, J-F., University of Liege (Belgium) | Melo de Sousa, N., University of Liege (Belgium) | Indrova, E., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic) | Lopatarova, M., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic) | Dolezel, R., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic) | Cech, S., University of Veterinary and Pharmaceutical Sciences, Brno (Czech Republic)
The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to desensitise gonadotroph cells of the pituitary gland. Thereafter, follicular development was stimulated by exogenous FSH treatment. Intrafollicular treatment with 10, 5, 1 and 0.01 microg LH was performed on one single follicle while other follicles remained untreated. Human chorionic gonadotrophine (2000 UI) was administered intravenously as a control. Ovulation and development of the corpus luteum occurred after all intrafollicular treatments with 10 and 5 microg LH. After IFT using 1 microg of LH 75% animals (3/4) ovulated. The dose of 0.01 microg was not followed by any ovulation whereas control treatments with hCG were followed by an ovulation of the majority of follicles present in the ovaries. In conclusion, IFT with different doses of LH (greater than 0.01 microg) is capable of inducing ovulation.
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