Development of molecular markers associated with enhancement of some quality traits in Sugarcane saccharum spp.
2011
Khaled, S.K.M.
The aim of this study was to detect some molecular markers associated with sugar content in sugarcane using DNA-based PCR techniques. To assist selection of high sugar content promising lines at a very early stage of the sugarcane breeding program; RAPD,SSRs and R-ISSRs techniques were used for detecting markers associated with sugar content trait. The performances of twenty two sugarcane clones revealed that RAPD, SSRs, ISSRs and R-ISSRs techniques are useful as marker assisted selection for sugar content trait in these clones. Twenty two positive and ten negative markers were obtained using RAPD-PCR analysis.Eight positive and five negative markers were obtaind using ISSRs-PCR analysis while using R-ISSRs PCR analysis produced 28 positive markers and 17 negative markers which could use as marker assisted selection for sugar content. Using sorghum SSR primers revealed six positive and seven negative markers. The study confirmed that R-ISSRs technique was more effective analysis and a higher resolution results.
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