Molecular cloning and expression studies of a full length c-dna coding for trehalose 6-phosphate from Egyptian durum wheat
2011
El-Said Hassan, L.S.D.
In developing countries dehydration stress is considered as one of the major constraints for food production. The severity of symptoms associated with the dehydration stress can range from grain weight to reduction in the crop yield. This will have a great impact on major crops such as wheat which is considered to be the firrst ranked important cereal crop. A series of experiments were conducted on the leaves of Durum wheat sohag variety 3 under normal and dehydration shock stress conditions (2,4,6 hours) in order to examine the magnitude of the TPP gene response to dehydratlon. The full length of TPP gene was isolated by RACE-PCR yielding a length of 1458 bp. Bioinformatics analysis was used in order to perform a profiling for the TPP gene expression after being isolated by RT-PCR. Aphylogentic analysis was performed to relate the closest species to the wheat revealing the Oryza sativa which also shown similarity in the comparative mapping revealing that the TPP gene sequence in the Durum wheat to be found on the Oryza sativa on chromosome 8 . The crystallization of the TPP protien strcture revealed another characteristic for the Trehalose being an affective membrane stabilizer In vivo and in vitro. The Bioinformatics analysis and the conducted experiment confers that the TPP level increased under the dehydration stress indicating an up regulation for the gene and proving to be a membrane stabilizer.
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