Development of an Effective Extraction Method for the Quality Control of Eriobotrae Folium : Determination of Triterpenic Acids
2010
Lee, K.I., Biotechnology Industrialization Center, Dongshin University, Naju, Republic of Korea | Park, M.Y., Jeollanamdo Agricultural Research and Extension Service, Haenam, Republic of Korea | Pyo, B.S., Dongshin University, Naju, Republic of Korea | Kim, S.M., Dongshin University, Naju, Republic of Korea
In result of analysis for the content determination of ursolic acid(UA) and oleanolic acid(OA) in extract of Eriobotryae folium using HPLC with UV detector, UA in chloroform extract of Moo-mok variety was showed highest content(2.7843 mg/g). And OA in ethyl acetate extract of Dae-bang variety was showed highest content(0.5898 mg/g). These result suggest that direct extraction using organic solvent(chloroform or ethyl acetate) was useful method for rapid quantitative analysis of UA and OA without preprocessing such as drying or fractionation.
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