Influence of genotype and harvest time on the phenolic content of horseradish (Armoracia rusticana L.) roots
2012
Tomsone, L., Latvia Univ. of Agriculture, Jelgava (Latvia) | Kruma, Z., Latvia Univ. of Agriculture, Jelgava (Latvia) | Lepse, L., Pure Horticultural Research Centre, Tukuma reg. (Latvia)
Horseradish (Armoracia rusticana L.) is a perennial plant, with a particularly pungent flavour and significant antioxidant properties. The aim of current research was to determine the total phenol content and antioxidant properties of horseradish depending on genotype and harvest time. For experiments nine genotypes of horseradish roots collected at different times were investigated. Fresh plant material was extracted with ethanol/water solution (80:20 v/v). Total phenols content (TPC) of plant extracts was determined according to the Folin-Ciocalteu spectrophotometric method and results were expressed as gallic acid equivalents (GAE). Antioxidant activity of the extracts was measured on the basis of DPPH˙ free radical scavenging activity and the final results were expressed as inhibition of 2,2-diphenyl-1- picrylhydraziyl (DPPH˙) in percent (I, %). Total phenols content varied among analysed types of horseradish. The highest TPC was detected in horseradish root genotype 280 harvested in September and it also demonstrated the highest DPPH˙ radical scavenging activity, whereas the lowest TPC was detected in horseradish root genotype 26B also harvested in September. TPC and DPPH˙ scavenging antioxidant activity were also significantly influenced by harvest time. Positive correlation was found between antiradical activity and the total phenols content in horseradish roots harvested in September. In further experiments, use of horseradish as natural antioxidants in different food matrixes should be studied.
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