Detection of Orf virus and papillomavirus out of samples from goats and cattle which were gathered by multiple PCR Romania

English. The lesions which appear on the skin and are caused by parapoxvirus, herpesviruses and papillomavirus, depending on they’re gravity, can produce large economic losses. The identification of viral species with different laboratory methods can facilitate making a correct diagnostic in identifying the infectious entity. The most common encountered injuries, especially on goats and kids, have an oral location, breasts, eyes and genital and podal. On the other side on cattles infected with papillomavirus the lesions are multiple and spread all over the body. To identify the etiologic agent from goats and cattle with skin lesions, tissue samples were collected and scabs. The virus identification was made with molecural biology techniques. The PCR method revealed the viral DNA, thus identifying a portion of the B2L preserved gene which is then amplified using primers PPP1-PPP4. For papillomavirus detection, primers FAP59 and FAP 64 were used to conserve the L1 human gene, primers that can help detect other types of papillomavirus from other species. The PCR results showed that lesions from goats were made by Orf virus, a virus that belongs to the papillomavirus gene, as for cattles the bovine papillomavirus. Studies have shown that PCR is a rapid laboratory method which can be used to identify the viral agent that causes severe skin lesions.

Romanian; Moldavian; Moldovan. Leziunile care apar la nivelul pielii, determinate de parapoxvirusuri, herpesvirusuri si papillomavirusuri, in functie de gravitatatea lor, pot produce mari pierderi de natura economica. Identificarea speciilor virale prin diferite metode de laborator poate usura, astfel, punerea unui diagnostic precis in identificarea entitatilor infectioase. Cele mai frecvente leziuni intilnite, in special la capre si iezi, au localizare bucala, peribucala, mamara, oculara, podala si genitala. In schimb, la bovinele infectate cu papillomavirus, leziunile sunt multiple si dispersate pe tot corpul. Pentru identificarea agentului etiologic de la capre si bovine cu leziuni la nivelul pielii s-au recoltat probe de tesut si cruste. Identificarea virusului s-a facut prin tehnici de biologie moleculara. ADN viral a fost pus in evidenta prin metoda PCR, identificandu-se, astfel, o portiune a genei conservate B2L, amplificata, apoi, cu ajutorul primerilor PPP1 _ PPP4. Pentru detectarea papillomavirusului s-au folosit primerii FAP59 si FAP64, utilizati pentru conservarea genei L1 de tip uman, primeri cu care se pot detecta si alte tipuri de papillomavirus de la alte specii. Rezultatele obtinute in urma PCR-ului si a secventializarii au demonstrat ca leziunile de la capre au fost produse de virusul Orf, virus ce face parte din genul Parapoxvirus, iar la bovine de Bovine papillomavirus. Studiile au demonstrat ca PCR este o metoda de laborator rapida si poate fi utilizata pentru identificarea agentului viral ce determina leziuni grave la nivelul pielii.