Association of Colony Morphology with Coenzyme Q∧10 Production and Its Enhancement from Rhizobium radiobacter T6102W by Addition of Isopentenyl Alcohol as a Precursor
2012
Seo, M.J., Korea Food Research Institute, Sungnam, Republic of Korea | Kook, M.C., Anyang University, Incheon, Republic of Korea | Kim, S.O., Union Korea Pharm., Co. Ltd., Seoul, Republic of Korea
Rhizobium rudiobucter T6102 was morphologically purified by the aniline blue agar plates to give two distinct colonies; white smooth mucoid colony (T6102W) and blue rough colony (T6102B). The coenzyme Q∧10 (CoQ∧10) was produced just by T6102W, showing 2.0 mg/g of CoQ∧10 content, whereas the T6102B did not produce the CoQ∧10. All of the used CoQ∧10 biosynthetic precursors enhanced the CoQ∧10 production by T6102W. Specifically, the supplementation of 0.75 mM isopentenyl alcohol improved the CoQ∧10, concentration (19.9 mg/l) and content (2.4 mg/g) by 42% and 40%, respectively.
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