Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea
2012
Youm, J.Y., Korea National University of Education, Cheongwon, Republic of Korea | Han, H.K., Korea National University of Education, Cheongwon, Republic of Korea | Chung, J.M., Korea National Arboretum, Pocheon, Republic of Korea | Cho, Y.C., Korea National Arboretum, Pocheon, Republic of Korea | Lee, B.C., Korea National Arboretum, Pocheon, Republic of Korea | Eom, A.H., Korea National University of Education, Cheongwon, Republic of Korea
This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.
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