Shoot proliferation of banana cv. grande naine through horizontal sectioning of the shoot tip
2010
Mahdi Safarpour | Uma Rani Sinniah, Universiti Putra Malaysia (Malaysia). Dept. of Crop Science | Sreeramanan Subramanian, Universiti Sains Malaysia (Malaysia). School of Biological Sciences | Maheran Abdul Aziz, Universiti Putra Malaysia (Malaysia). Dept. of Agriculture Technology
Cavendish (AAA genome type) is one of the most important banana cultivar with about 47% global production (Arias et al., 2003). The edible bananas and plantains are conventionally propagated through vegetative means using sword suckers which is very slow. The in vitro method is used for mass production. Here, an improved technique for rapid multiplication for large scale micropropagation was reported. The aim of this study was to establish an applicable protocol for shoot proliferation through horizontal sectioning of the individual shoot tip using suckers of banana cv. Grande Nain (Musa spp. AAA group). The excised shoot tips were cultured on MS medium supplemented for one month. After 2 months, the multiple shoots which emerged from each segment were isolated and cultured on MS medium supplemented with three different concenrations of BP (10, 20, 30 uM) for further multiplication and determination of the best BAP concentration for shoot proliferation with monthly subculture interval. As a control the excised shoot tips were cultured on MS medium supplemented with 10, 20 and 30 uM, BAP, with one month subculture interval for six month without sectioning. All experiments were arranged in CRD [Complete Randomized Design] with three replications with 10 explants per replication per treatment. Parameters observed were the number of shoots produced per explants per subculture. The horizontal sections of cv. Grande Naine placed onto MS medium containing 10 uM BAP resulted in high multiple shoot production. The multiple shoots obtained in this step were isolated and cultured on MS medium supplemented with 10, 20 and uM of BAP for further shoot proliferation. The mean number of proliferated shoots obtained at 10 and 30 uM of BAP was 3.53 and 4.12 after four subcultures respectively. The number of proliferated shoots can be maximized by using horizontal sections of the individual shoot tip resulting in 49% increase.
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