Differential expression of cytosolic and membrane associated proteins in different rat brain regions
2011
Zahid, S. (Karachi Univ. (Pakistan). Dept. of Biochemistry) | Khan, R. (Karachi Univ. (Pakistan). Dept. of Biochemistry) | Dar, A. (Karachi Univ. (Pakistan). HEJ Research Inst. of Chemistry) | Azhar, A. (Karachi Univ. (Pakistan). The Karachi Inst. of Biotechnology and Genetic Engineering) | Ahmed, N.
The present research focuses on the expression profiling of brain regional membrane proteins and expression changes within two rat brain regions hippocampus and cortex. The protein extracts from rat brain regions (cortex n=5 and hippocampus n=5) were collected through subcellular fractionation, which allows the analysis of proteins in their physiologic and intracellular context. High-resolution maps of the hippocampal and cortex proteome were generated through sodium dodecyl sulphate polyacry Jamide gel electrophoresis (SDS-PAGE) and the protein spots were visualized with coomassie brilliant blue and silver staining methods. A 69, 58, 45, 35 and 25 kDa protein shows more than 2 fold up regulation in cortex membrane fraction as compared to hippocampus membrane fraction while increased expression was observed for 58, 55, 45, 35, 29 and 25 kDa proteins in cytosolic fraction of hippocampus in comparison to cortex. Further, protein components of 63, 55, 25 and 21 kDa have shown negligible expression in hippocampus membrane fraction. Moreover, a 50 kDa protein component is only expressed in cortex membrane and cytosolic fraction with decrease expression in the both hippocampal fractions. The restraining of these membrane proteins to a particular region provides clues about the involvement of proteins in catalyzing particular functions of each brain region. Moreover, the expression profiles of membrane and cytosolic proteins will help in elucidating the functional role not only in the particular region but also in a specified organelle. Further characterization through mass spectrometry will be helpful in identification and elucidation of the biological role of the expressed proteins.
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