Investigations on mass production of the potato rot nematode, Ditylenchus destructor (Thorne, 1945) using different monoxenic cultures
2013
Namjou, S. (Agricultural Organization, Kerman (Iran). Plant Protection Dept.) | Shokoohi, E. (Shahid Bahonar Univ. of Kerman (Iran). Plant Protection Dept.) | Lori, Z. (Shahid Bahonar Univ. of Kerman (Iran). Plant Protection Dept.)
The reproduction of an Iranian population of Ditylenchus destructor Thorne, 1945 was investigated in different media under laboratory conditions to identify the best medium and fungal host for mass production of this nematode. Four media, including three handmade PDA media (Potato Dextrose Agar), prepared from extracts of three cultivars of potato (Santana, Ramos & Maradona) and one medium of OMA (Oat Meal Agar), were used as substrates to grow fungi isolated from potato fields, including different anatomizes group of Rhizoctonia solani (AG3, AG4, AG5, AG6, AGD, AG9), Phytophthora capsici, Botrytis cinerea and Fusarium oxysporum f.sp. lycopersici. These fungal cultures were then inoculated with the nematodes and maintained at 20 and 25 degree C. The results showed that the nematode fed and reproduced more on R. solani (AG3, AG4 and AGD) and F. oxysporum f.sp. lycopersici than on other fungi. However, R. solani (AG3 and AG5) and P. capsici at 25 degree C were also suitable for mass production of this nematode. The reproduction of the nematode was greater at 25 degree C than at 20 degree C.
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