Determination of Cyanidin-3-Glucoside Content using Visible/Near Infrared Reflectance Spectroscopy (VIS/NIRS) in Black Rice
2012
Lee, J.S., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Kwak, D.Y., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Park, N.B., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Song, Y.C., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Oh, S.K., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Lee, J.H., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Yoon, M.R., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Kim, H.W., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Kwak, J.E., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Kim, C.K., National Institute of Crop Science, RDA, Suwon, Republic of Korea | Hwang, Y.S., Kangwon National University, Samcheok, Republic of Korea | Choung, M.G., Kangwon National University, Samcheok, Republic of Korea
Brown rice grain pigments of black rice have a higher content of bioactive substances such as anti-mutagenic substance than the non-pigmented rice grain. The major anthocyanin pigment contained in black rice was cyanidin-3-glucoside. This study was conducted to establish a rapid analysis method for determining cyanidin-3-glucoside contents in flour and whole rice seeds of black rice using VIS/NIRS technique. A total of 60 black rice samples were used for VIS/NIRS equation model development and validation. The value of coefficient of determination of external validation (r²) and standard error of performance (SEP) in whole rice seed sample were 0.653 and 97.2, respectively. Therefore, the value of it seemed to be difficult to analyze cyanidin-3-glucoside content in whole rice seed samples using VIS/NIRS. However, in rice flour sample, the best accurate equation model was obtained from the partial least square regression (PLS) method. The value of r², SEP and bias were 22.5, 0.922 and -1.45 in the calibration transformed to the N-point smooth of log 1/R signal, 5 factors, respectively. Therefore, the results of our study clearly demonstrate that the VIS/NIRS method would be applicable only for rapid determination of cyanidin-3-glucose content in black rice flour samples.
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