Evaluation of growth characteristics and genetic diversity of commercial and stored lines of Hypsizygus marmoreus
2013
Qiu, C. (South China Univ. of Technology, Guangzhou (China). School of Bioscience and Bioengineering) | Yan, W. (Guangdong Inst. of Microbiology, Guangzhou (China). Applied Microbiology) | Li, P. (South China Univ. of Technology, Guangzhou (China). School of Bioscience and Bioengineering) | Deng, W. | Song, B. | Li, T.
Hypsizygus marmoreus is an edible mushroom of commercial importance with medicinal properties. Therefore, cultured commercial and stored strains of the fungus were evaluated for various cultivation characteristics (hyphal growth, and size/morphology of fruiting body), and genetic diversity among the strains by inter-simple sequence repeat (ISSR) analysis. We collected 32 H. marmoreus strains from different locations in China, and cultured them at the Gumuzhen Bio-technology facility. The cultivation characteristics differed among the main H. marmoreus strains. The genomic DNA extracted from H. marmoreus was amplified with 18 ISSR primers, which generated 199 bands The average number of amplified bands per primer was 11.10 (mean polymorphism 87.4%). The polymorphism information content (PIC) value for ISSRs ranged from 0.39 to 0.50 (mean PIC value per ISSR 0.48), indicating a medium level of polymorphism among the strains. The analysis of cultivation characteristics and ISSR data showed that there was rich diversity among the strains of H. marmoreus. A cluster analysis based on ISSR results classified the 32 of strains of H. marmoreus into three main groups. These results showed that ISSR is a useful tool for selecting potential strains and predicting hybrid performance in the out breeding of mushrooms.
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