Induction of probiotic strain Enterococcus faecium EF1 on the production of cytokines, superoxide anion and prostaglandin F2 in a macrophage cell line
2012
Wei-fen, L. (Zhejiang Univ., Hangzhou (China). Coll. of Animal Science) | Huang, Q. (Zhejiang Univ., Hangzhou (China). Coll. of Animal Science) | Ya-li, L. (Zhejiang Univ., Hangzhou (China). Coll. of Animal Science) | Rajput, I.R. (Zhejiang Univ., Hangzhou (China). Coll. of Animal Science) | Huang, Y. (Guangxi Univ., Nanning (China). Coll. of Animal Science and Technology) | Cai-hong, H.
Immunological effects of probiotic strain Enterococcus faecium EF1 was evaluated through the production of cytokines, superoxide anion (O2) and prostaglandin E2 (PGE2) in murine macrophage cell line RAW 264.7. In addition, the responses were contrasted with a pathogenic coliform, Escherichia coli strain K88. After 12-h co-incubation of RAW 264.7 with E. faecium EFI or E. coli K88, the culture supernatants were collected for further analysis of cytokines (TNF-alpha, IFN-gamma, IL-1 beta, IL-6, IL-12, IL-8 and IL-10) and inflammatory mediators including superoxide anion (O2) and PGE2. The results showed that, E. faecium EFI induced an increased (P less than 0.01) release of TNF-alpha, IFN-gamma, IL-6, IL-10 and O2 in RAW 264.7 cells. However, levels of cytokines induced by E. faecium EFI were far lower (P less than 0.01) than those observed following co-culture with E. coli K88. In addition, when RAW 264.7 cells were first treated with E. faecium EFI and then infected with E. coli K88, the production of IL-12 and O2 in response to E. coli K88 were significantly suppressed, indicating that E. faecium EFI may limit the ability of macrophages to induce excessive inflammation even to potent inflammatory bacteria. Our current findings concluded that, E. faecium EFI was capable of triggering a moderate innate inflammatory response on direct contact with murine macrophage cell line RAW 264.7.
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