Effect of Plant Growth Regulators on Chloroplast Ultra Structure in Lamium Album Plantlets
2013
Stefanova, M., Sofia University St. Kliment Ohridski, Sofia (Bulgaria) | Koleva, D., Sofia University St. Kliment Ohridski, Sofia (Bulgaria) | Ganeva, T., Sofia University St. Kliment Ohridski, Sofia (Bulgaria)
Lamium album L. stem nodal segments were grown on Murashige and Skoogs medium without growth regulators control and on media supplemented with 0.2 mg l-1 N6-benzyladenine, 0.5 mg l-1 indole-3-butyric acid or both plant growth regulators. The plastid apparatus of in vitro formed leaflets was studied by transmission electron microscopy. The cytokinin N6-benzyladenine negatively affected the chloroplast formation when was applied individually or in combination with the auxin indole-3-butyric acid. On the contrary, IBA in concentration of 0.5 mg l-1 was appropriate for development of chloroplasts with regular shape and well organized internal membrane system thus providing for the regeneration capacity in vitro.
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