Effects of Exogenous Substance on Starch Synthesis and Related Enzymes Activity in Chinese Chestnut | 外源物质对板栗淀粉合成及相关酶活性的影响
2013
Guo Sujuan, Beijing Forestry University, Beijing(China),Key Laboratory for Silviculture and Conservation of Ministry of Education | Xie Peng, Beijing Forestry University, Beijing(China),Key Laboratory for Silviculture and Conservation of Ministry of Education
Chinese. 糖分的积累是果实品质形成的关键,蔗糖不仅为植物的生长发育提供物质和能量,也可作为一种信号物质调节碳水化合物代谢和控制同化物分配,进而影响植物的生长发育（谢虹,2003;陈俊伟等,2002）。许多学者从蔗糖代谢相关酶的活性变化来探讨果实糖积累的机制 。
Show more [+] Less [-]English. In this research, we sprayed 0.3% calcium chloride, 5 mmol L-1 EGTA, 4% sucrose solution on the surface of leaves to explore the interactions of the total soluble sugar, sucrose, and starch content as well as the relevant enzyme activity of Chinese chestnut ＂yan shan zao feng＂ in the fruit establishment stage. The results indicated that： the sprayed sucrose solution increased soluble sugar, sucrose, and starch content, extended the duration of starch accumulation, and promoted sucrose synthase （SS）, sucrose phosphate synthase （SPS）, ADPG Pyrophosphorylase （AGPPase）, soluble starch synthase （SSS） and granule-bound starch synthase （GBSS） activities. Individual fruit weight increased by 5.15%. CaCl2 showed no significant effect on growth and development indexes of Chinese chestnut, while EGTA treatment reduced the single fruit weight, disturbed the Chinese chestnut kernel development amd caused formation of Empty-shell, inhibited the activity of SS, SPS, AGPPase, SSS and GBSS, and reduced the soluble sugar, sucrose, starch content. Spraying sucrose solution during the Fruit Growth and Development of Chinese chestnut is one of operational measurements.
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