Relationship between the Lepidosaphes salicina-Caused Damages and Activities of Leaf Defensive Proteins of Syringa spp. | 柳蛎盾蚧危害与丁香防御蛋白活力的关系

Chinese. 在柳蛎盾蚧的未危害期（5月末）、危害盛期（6月末）、危害弱期（7月末）、危害末期（8月末）,测定高抗类[什锦丁香等7种（品种）]、中抗类[西南丁香等3种（品种）]、易感类[紫丁香等2种]、高感类（红丁香）13种（品种）丁香叶中的POD,SOD,CAT,PPO,PAL,TI和CI 7种防御蛋白的活性.结果表明：危害时期,丁香种（品种）对丁香防御蛋白活力有极显著影响（P＜0.01）.POD活性,在危害盛期,在高抗、中抗类丁香中均显著升高（P＜0.05）,而在易感、高感类中应激滞后,即分别在危害弱期和危害末期升高.CAT在易感类和高感类丁香防御上起主导作用,其活性在危害盛期显著升高,而中抗和高抗类未见明显变化.SOD活性,在危害盛期高抗类丁香中无显著变化,而其余3类则显著降低.PPO活性在未危害期的高低与抗性相关,在虫害盛期诱导表达的强弱也与抗性相关;PAL活性未能反映抗性品种间的差异.TI和CI活性,在未危害期的高低与其抗虫性无明显相关性,在危害盛期的升高幅度与抗虫性呈正相关.综上所述,PPO在未危害期的活力可作为筛选丁香抗虫种（品种）的指标,危害盛期POD,PPO,TI和CI活性升高的幅度与丁香抗虫性呈正相关。

English. In this study,we investigated the relationship between activities of various plant defensive proteins and pest-resistance to the Lepidosaphes salicina by testing defensive activities of seven proteins （POD,SOD,CAT,PPO,PAL,TI and CI） from leaves of 13 Syringa species （seven species with high pest-resistance,three species with intermediate resistance,two species with susceptibility and one with very susceptibility） in different pest periods.The results showed that there was highly significant difference in defensive proteins activities among different Syringa species （P ＜ 0.01） in the pest period.POD activity of the species with high and intermediate pest-resistance was significantly increased （P ＜0.05） in the peak-infesting period （by the end of June）.However,POD activity of the species with susceptibility and very susceptibility was increased in the weak-infesting period （by the end of July） and the late-infesting period （the end of August）,respectively.CAT activity in susceptible and very susceptible species was significantly increased in the peak-infesting period,and the enzyme might play a primary role in protection of the species from pest damage.SOD activity in the high resistant species had no change,while it in other species was significantly decreased in the peak-infesting period.The PPO activity in the pre-infesting period （by the end of May） was positively correlated with the resistance and its activity in the peak-infesting period also had correlation with pest resistance.There were no differences in PAL activity between different resistance species.Similarly no significant correlation was found between the activities of TI and CI in the pre-infesting period and the pest resistance.In summary,the level of PPO activity in the pre-infesting period is recommended as an index for screening the resistant species.Increases in POD,PPO,TI and CI activities in the peak-infesting period were positively correlated with pest-resistance level of Syringa spp.