Cloning and expression analysis of a disease resistant gene VaAC1 in adzuki bean | 小豆抗病相关基因VaAC1的克隆与表达分析
2013
Chen Xin, Nanjing Agricultural University,Nanjing(China),College of Agriculture | Chen Huatao, Jiangsu Academy of Agricultural Sciences,Nanjing（China），Institute of Vegetable Crops | Wan Jianmin, Nanjing Agricultural University,Nanjing(China),College of Agriculture
Chinese. 利用简并引物从小豆中扩增出1条与植物几丁质酶基因高度同源的片段,命名为VaAC1,通过RACE技术延伸VaAC1基因的3＇端和5＇端,VaAC1基因完整ORF长度为885 bp,编码294个氨基酸,拥有几丁质酶蛋白家族典型保守结构域GH18_chitinase-like。RT-PCR分析表明,VaAC1基因在小豆根、茎和叶中均有表达,且在叶中的表达相对较高;大豆花叶病毒处理后,VaAC1基因在叶中的表达量显著增强,显示小豆VaAC1基因与病毒病密切相关。
Show more [+] Less [-]English. In this study,a chitinase homologue gene was cloned using degenerate primers in adzuki bean,and the gene was named VaAC1.The 3′ end and 5′ end sequences of VaAC1 gene were extended by RACE method.As a result,the complete ORF of VaAC1 gene was 885 bp,encoding 294 amino acids.VaAC1 had a typical conserved domain GH18_chitinase-like of the chitinase protein family.Expressions of VaAC1 gene were detected in root,stem and leaf using RT-PCR method in adzuki bean,and the expression of VaAC1 gene in leaf was higher than in root and stem.The expression of VaAC1 gene in leaf was significantly enhanced after Soybean mosaic virus treatment,which revealed the close relationship between VaAC1 gene and virus disease resistance in adzuki bean.
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