Effects of intranasal immunization with attenuated Mycoplasma hyopneumoniae strain in combination with adjuvant on the antibody secreting cells in pig respiratory tract | 猪肺炎支原体弱毒株与佐剂配合鼻腔免疫对仔猪呼吸道抗体分泌细胞的影响

Chinese. 应用猪肺炎支原体弱毒株配合佐剂CpG鼻腔免疫仔猪,检测呼吸道（鼻黏膜、气管和肺）IgA和IgG分泌细胞的分布与面积变化。30头仔猪随机均分为5组,7日龄首免,鼻腔免疫组10日龄二免,首免后42 d宰杀,取鼻黏膜、气管和肺,利用免疫组化技术检测IgA和IgG分泌细胞。结果表明：应用猪肺炎支原体弱毒株配合CpG鼻腔免疫后6周,鼻黏膜IgA和IgG分泌细胞的面积均极显著增加（P〈0.01）,气管IgA分泌细胞的面积也显著增加（P〈0.01）。而单独应用猪肺炎支原体弱毒株鼻腔免疫或肌肉注射虽然也能显著增加鼻黏膜和气管IgA、IgG分泌细胞的面积（P〈0.01）,但仍显著低于配合佐剂鼻腔免疫的效果。在肺中并未检测到IgA和IgG分泌细胞。结果提示,猪肺炎支原体弱毒株配合CpG鼻腔免疫能够增强局部呼吸道体液免疫应答水平。

English. This experiment was conducted to investigate the area changes of IgA and IgG secreting cells in respiratory tract （mainly nasal mucosa, trachea and lung ）of piglets after intranasal immunization with attenuated Mycoplasma hyopneumoniae strain in combination with CpG. 30 newborn piglets were randomly divided into 5 groups, and on being 7 days old, each pig was immunized by intramuscular and intranasal routes. The pigs from intranasal immunization group were immunized again 3 days later. The nasal mucosa, trachea and lung were collected at 42 day-post immunization after the pigs were slaughtered. The antibody secreting cells were showed by immunohistochemieal technology. Resalts showed that the areas of IgA and IgG secreting cells in nasal mucosa and IgA secreting cells in trachea increased significantly after intranasal immunization with attenuated M. hyopneumoniae strain coupled with adjuvant CpG（P〈0. 01 ）. Also the increased areas were found in the pigs from intranasal immunization with attenuated M. hyopneumoniae strain alone and intramuscular immunization group （P〈0.01） , but the effect was much unsatisfied when compared to the attenuated M. hyopneumoniae strain coupled with adjuvant group. No IgA and IgG secreting cells in lung were detected. The results demonstrated that intranasal immunization with attenuated M. hyopneumoniae strain coupled with adjuvant CpG could elicit local immune response in the respiratory tract.