Effects of elevated CO2 on food selection and energy storage of Mythimna separata （Walker） （ Lepidoptera ： Noctuidae） | CO2浓度升高对黏虫的取食选择和能量贮存的影响

Chinese. 采用选择性和非选择性方法测定了黏虫幼虫对高CO2浓度下生长的小麦叶片的选择性；并观察了在高CO：浓度下连续生长至第7代后的幼虫存活情况，测定了老熟幼虫体内保护酶活性以及成虫能源贮存。结果表明：在非选和可选情况下，3龄和4龄幼虫偏好当前CO2（390μL・L. 1）条件下生长的小麦叶片，取食量比高CO2（780μL・L. 1）条件下提高26．7％和30．3％（非选择性测定）或是高CO2下的4．2倍和1．6倍（选择性测定），6龄幼虫未表现出显著的取食偏好。高CO2处理下连续饲养至第7代的幼虫孵化至化蛹的存活率显著低于当前CO2处理，主要差异表现在4龄之后，但老熟幼虫体内的保护酶活性和蛋白质含量在当前和升高CO2处理之间没有显著差异。升高CO2处理可显著降低第7代成虫甘油i酯含量和体干质量，使体内水分含量明显增多；但对肌肉干质量和糖原无显著影响。上述结果说明：CO2浓度升高对黏虫幼虫存活和成虫迁飞所需能量贮存具有不利影响。

English. In this study non-choice and dual-choice trials were conducted in the laboratory by measuring food consumption of leaves clipped from the wheat cultured in incubators under either ambient （390 μL・L. 1 ） or elevated （780 μL・L. 1） CO2. To evaluate cumulative effects of elevated CO2 on larval survival, the 7th generation under elevated CO2 was observed in larval survival, activity of protection enzymes, and energy storage. The L3 and L4 worms showed obvious selection for wheat leaves from the ambient CO2 as compared to those from the elevated CO2 ,with increasing leaf consumption by 26.7% and 30.3% （non-choice） or as much as 4.2 and 1.6 times（dual-choice） , respectively. But the L6 did not exhibit the choice. The survival from larva hatching to pupation in the 7th generation was lower for the larvae fed on wheat from the elevated CO2 treatment than on wheat from the ambient CO2. There were, however, no significant differences in activity of protection enzymes and protein contents examined between CO2 treatments. The elevated CO2 reduced triglyceride content and body weight but increased water content, in adults of the 7th generation. Glycogen and muscle,however,were not significantly influenced by elevated CO2 treatment. The results of this study suggested that elevated CO2 has negative effects on larval survival and energy storage of Mythimna separata（Walker）.