Protective effects of compound ammonium glycyrrhizin soluble powder on liver injury induced by LPS and enrofloxacin in chickens | 复方甘草酸单铵可溶性粉对恩诺沙星联合LPS致鸡肝损伤保护机制研究

Chinese. 104羽海蓝蛋鸡随机分为空白组（I）、模型组（Ⅱ）、复方甘草酸单铵可溶性粉（CAG）预防组（Ⅲ）、CAG治疗高剂量组（IV）和CAG治疗低剂量组（V），除I组外，各组连续灌服恩诺沙星100mg・kg. 1，1次・d. 1，第3天灌药同时腹腔注射脂多糖粗提液（LPS）4mL・kg. 1以建模。Ⅲ组在灌服恩诺沙星前3d混合饮用CAG40mg・L. 1至建模止；IV、V组腹腔注射LPS同时分别灌服22．5和7．5mg・kg～CAG，2次・d. 1，重复3d。各组均在腹腔注射LPS后分别于6、24和48h采血，检测血清生化指标，观察肝组织病理学变化。试验结果如下：Ⅱ组鸡血清ALT、AST、γ-GT、TBIL、MDA和NO水平在6、24和48h均显著高于I组，而TP、ALB、SOD和GSH均显著低于I组。与Ⅱ组相比，Ⅲ组各指标多在6和24h差异显著，只MDA、ALB和GSH持续至48h仍显著，Ⅳ组各指标均在24和48h差异显著，V组各指标差异均不显著。仅Ⅳ组DBIL浓度在24和48h较I组显著升高，其余组无显著差异；IV组较其他组鸡肝细胞病变改善最大。结论：在自由基损伤介导肝损伤过程中，CAG有一定保护作用，效果受给药时机和剂量影响。

English. A total of 104 chickens was divided randomly into 5 groups：Group I served as normal control. Group Ⅱ , Ⅲ, IV, and V were administered enrofloxacin（ 100 mg・kg. 1） once a day, and on the 3rd day, administered lipopolysaceharide crude extract（LPS） （4 mL・ kg. 1 ） at the same time. Group Ⅱ was maintained as liver injury control. Group Ill received preventive treatment of compound ammonium glycyrrhizin soluble powder（CAG） in drinking water at the dose of 40 mg・L. 1（ by ammonium glycyrrhizin, the same as follows） for three days before enrofloxaein treatment, and till to LPS treatment. Group IV and V received CAG at the dose of 22.5 and 7.5 mg・kg. 1, twice a day for three days after LPS treatment. Eight chickens were sacrificed at 6,24,48 h post-treatment with LPS and their blood sample were harvested for detection of serum biochemical indexes. Their livers were dissected out and evaluated for histopathological changes. Result ： at 6,24,48 h post-treatment with LPS, the levels of serum ALT, AST, γ-GT, TBIL, MDA and NO were significantly higher, and TP, ALB, SOD and GSH were significantly lower in Group II than in Group I . Compared to Group Ⅱ , in Group m ,there was significant improvment of most indicators at 6 h and 24 h,but MDA,ALB and GSH at 6,24 and 48 h;in group IV, there was significantly improvment of all indicators at 24 h and 48 h. There was no obvious alteration were still significantly higher in Group V. There was significantly higher improvement in DBIL at 24 h and 48 h in Group IV than in Group I , no obvious alteration in others. The results of pathological study also support the results of biochemical findings. Conclusion ： the liver injury effect might be related to oxygen free radicals. CAG treatment could reduce such liver injury, probably by its antioxidant property.