Effects of polyphenols on the nitrite scavenging and N-nitrosamine formation in simulated gastric acid | 植物多酚对模拟胃酸体系中亚硝酸盐清除能力与亚硝胺生成的影响

Chinese. 在模拟胃酸体系中研究了茶多酚（TP）和葡萄籽提取物（GP）对亚硝酸盐清除能力和二乙基亚硝胺（NDEA）生成的影响,并对两种多酚的抗氧化性进行了分析。NDEA和亚硝酸盐含量分别采用液相色谱法和分光光度法进行测定。结果表明：随多酚浓度增加和反应时间延长,TP和GP对亚硝酸盐清除能力增强,其中TP的作用效果强于GP。随TP和GP添加量的增加,体系中NDEA相对生成量逐渐增加,当添加0.5 mg.mL-1TP和1.0 mg.mL-1GP时,NDEA相对生成量达到最大,此后NDEA的相对生成量逐渐减少,最终完全抑制了NDEA的形成。抗氧化活性分析表明,随多酚含量的增加,抗氧化能力增强,其中TP的抗氧化活性强于GP。结论：在本试验条件下,TP和GP具有较强的抗氧化和清除亚硝酸盐的能力,对NDEA的生成具有促进和抑制的双重作用效果。

English. In simulated gastric acid, effects of tea polyphenols （TP） and grape seed polyphenols （GP） on nitrite scavenging and nitrosamine formation were determined, and their antioxidant activities were assessed. The content of N-nitrosodiethylamine（NDEA） and nitrite were determined by high performance liquid chromatography （ HPLC ） and spectrophotometric method, respectively. The results showed that with plant polyphenols contents increasing and reaction times extending, the nitrite scavenging effect of TP and GP increased. The scavenging effect of TP was better than that of GP. With the increase of TP and GP contents, the relative content of NDEA increased gradually. When the contents of TP and GP were 0.5 and 1.0 mg.mL-1 ,the relative content of NDEA reached the maximum. Afterwards,the content of NDEA declined and finally the NDEA formation was inhibited completely. Antioxidant activity analysis showed that with the increase of polyphenols contents, the antioxidant capacity increased gradually. Also, Tp exhibited the higher antioxidant activity than GP. These results confirmed that at least in our experimental conditions, TP and GP had high nitrite scavenging and antioxidant ability, and possessed a dual role of facilitation and inhibition on NDEA formation.