Bioinformatics analysis of 4-hydroxy-3-methyl-2- buten1yl diphosphate synthase (HDS) in Populus Trichocarpa | 毛果杨1-羧基-2-甲基-2-（E）-丁烯基-4-二磷酸合酶（HDS）基因的生物信息学分析

Chinese. [目的]研究HDS基因间的同源性及其进化关系。[方法]以毛果杨的HDS基因为研究对象，利用生物信息学软件及网站对其进行碱基分布、氨基酸组成、亲疏水性、保守区以及二级结构和三级结构的预测与分析。并与其他10个物种的HDS基因序列进行多重比对与进化分析。[结果]毛果杨HDS基因的单链mRNA序列长1 956 bp，编码由656个氨基酸组成的蛋白质，该蛋白质相对分子质量为72 937.03，其中含量最高的是Leu，为10.50％；整个多肽中疏水性氨基酸只占39.63％，有10个亲水区和6个疏水区；与其他10个物种同源性多重比对发现同源性最高达99%。[结论]毛果杨HDS基因处于稳定状态，编码的蛋白为亲水性蛋白，在进化过程中是保守的；试验中获得的保守区段序列信息为新基因的克隆奠定了基础。

English. [Objective] The aim was to study on the homology and evolutionary relationship among HAD genes. [Method] Based on HDS gene of Populus Trichocarpa, bioinformatics software and website were used to predict and analyze base distribution, amino acids composition, relationship with water, hyperconservative region, secondary structure, and three-level structure. The results were used to compare with other HDS gene sequences of other ten species and related evolution analysis was followed. [Result] mRNA, single chain of HDS gene of Populus Trichocarpa, was 1 956 bp in length, code consisted of proteins made up of amino acid of 656, and the relative molecular mass was 72 937.03 with Leu as the highest of 10.50%; in polypeptide, hydrophobic amino acid was only of 39.63% with hydrophilc region of 10 and hydrophobic region of 6; after many comparisons with other ten species, homology was as high as 99%. [Conclusion] HDS gene of Populus Trichocarpa was quite stable and albumen of code was hydrophilic protein which was quite conservative in evolution; sequence information in hyperconservative region gained in the test paved the way for clone of novel gene.