Morphological analysis and gene mapping of a rice dwarf mutant | 一份水稻矮杆突变体的形态特征和基因定位
2012
Xie Hongjun1, Hunan Rice Research Institute, Changsha (China) | Tang Guohua (Zhan Qingcai), Hunan Rice Research Institute, Changsha (China) | Hunan Rice Research Institute, Changsha (China)
Chinese. [目的]对一株水稻矮杆突变体的形态特征进行分析，同时进行基因定位。[方法]以一株矮秆突变体（潇湘矮）为研究对象进行形态观察，并以潇湘矮作母本，半矮杆材料湘早143为父本，构建群体进行遗传分析，同时利用微卫星标记进行基因定位。[结果]潇湘矮的平均株高为55 cm；遗传分析结果显示所有F1株高偏向于父本, F2群体出现半矮杆植株和矮杆植株，比例接近于3∶1 性状分离, 证实潇湘矮株高性状受1对主效隐性基因控制；将该基因定位于水稻第5 染色体上，位于RM249上游, 遗传距离为8.4 cM。 [结论] rRH是一个新的水稻矮杆基因。
Show more [+] Less [-]English. [Objective] This study aimed to conduct morphological analysis and gene mapping of a rice dwarf mutant. [Method] A dwarf mutant (Xiaoxiang’ai) was used as test material for morphological observation. Xiaoxiang’ai was used as female parent and semi-dwarf material Xiangzao143 was used as male parent to construct populations for genetic analysis. Gene mapping was conducted by using micro-satellite markers. [Result] The average height of Xiaoxiang’ai was 55 cm; genetic analysis results showed that plant height of F1 individuals was similar with the male parent, while semi-dwarf plants and dwarf plants were observed in F2 populations and the segregation ratio was nearly 3:1, indicating that plant height trait of Xiaoxiang’ai was mainly controlled by one pair of recessive gene, which was located on rice chromosome 5 and in the upstream of RM249, with a genetic distance of 8.4 cM. [Conclusion] rRH is a new dwarf gene in rice.
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