A preliminary study on tissue culture and transgene of Casuarina equisetifolia L. | 细枝木麻黄组织培养和转基因研究
2012
Lin Yongsheng, Jiangkou Town Agricultural Service Center of Hanjiang District in Putian City of Fujian Province, Putian (China) | Jiang Jing, Chinese Academy of Forestry, Fuyang (China), Research Institute of Subtropical Forestry | Qiao Guirong, Chinese Academy of Forestry, Fuyang (China), Research Institute of Subtropical Forestry
Chinese. [目的]对细枝木麻黄进行组织培养和转基因研究。[方法]以耐寒性细枝木麻黄为试验材料，探讨了愈伤组织诱导、不定芽分化、农杆菌介导转化3种条件对转化效率的影响。[结果]对细枝木麻黄不定芽诱导分化适宜的激素组合为DCR+6-BA 5.0 mg/L+NAA 0.5 mg/L；转基因抗生素选择压力为潮霉素，共培养时间3 d；以初步建立的转基因体系利用农杆菌介导法获得94株转基因木麻黄，通过PCR检测，其中61株为PCR阳性植株。[结论]该研究为细枝木麻黄的组织培养和转基因研究奠定了基础。
Show more [+] Less [-]English. [Objective] The paper aimed to study the tissue culture and transgene of C. equisetifolia. [Method] C. equisetifolia were used as experimental materials to explore the effects of three conditions including callus induction, adventitious bud differentiation and Agrobacterium-mediated transformation on transformation rate of C. equisetifolia. [Result] The appropriate plant growth regulator combination on induction and differentiation of C. equisetifolia adventitious buds was DCR + 5.0 mg / L of 6-BA + 0.5 mg / L of NAA; hygromycin was selected for the selective pressure and co-culture time was 3 d; 94 stains of transgenic C. equisetifolia were obtained with the initially-established transgene system via Agrobacterium-mediated method, and 61 stains were PCR-positive plants according to the results of PCR detection. [Conclusion] The study had laid the foundation for tissue culture and transgene research of C. equisetifolia.
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