Effect of the acrylamide on the activity of p4502e1 in rat liver | 丙烯酰胺对大鼠肝脏P4502E1活力的影响

Chinese. 丙烯酰胺 (acrylamide, Acr) 具有毒性和潜在的致癌性, 食品中丙烯酰胺的存在及食用安全性引起了社会的高度重视。选用鼠龄50d的Sprague-Dawley雄性大鼠80只, 随机分成4组进行Acr染毒实验, 染毒剂量分别为0、25、50、100mg/kg,即对照组和低、中、高3个剂量组。染毒采用腹腔注射 (对照组使用生理盐水), 隔日染毒, 连续12周。在第3、6、9、12周末次染毒4h后处死取样。通过测定肝脏微粒体中细胞色素P4502E1 (cytochrome P4502El) 的活力来观察Acr对大鼠肝脏P4502E1活力的影响。结果表明,肝微粒体P4502E1活力在25mg/kg剂量组随染毒时间增加而增强, 染毒第12周的酶活力明显高于染毒第3、6、9周。在50、100mg/kg剂量组, P4502E1酶活力在染毒第6周出现峰值。研究P4502E1酶活力在肝脏丙烯酰胺代谢过程中的变化, 为进一步探讨丙烯酰胺代谢途径及其毒性作用奠定了基础。

English. Presence of acrylamide (Acr), which is toxic and potentially carcinogenic, in food and the resulting food safety has been paid a high attention. Eighty male Sprague-Dawley rats were selected, which were 180-220 g in body weight and 50 d old, and divided into four groups randomly to cunduct the contamination experiment by Acr. The contaminating dosages were 0, 25, 50, 100 mg/kg for control and low, middle and high groups, respectively. Intraperitoneal injection with Acr for experimental groups and physiological saline for control was done every other day for 12 weeks. Rats in each group were killed and sampled after 4 h since the last contaminating in 3 w, 6 w, 9 w, 12 w. The effect of Acr on the activity of cytochrome P4502E1 in liver were studyed by