Elderberry as a source of important flavonoid rutin
2011
Vespalcova, M., Vysoke Uceni Technicke, Brno (Czech Republic). Ustav Chemie Potravin a Biotechnologii | Grulichova, H., Vysoke Uceni Technicke, Brno (Czech Republic). Ustav Chemie Potravin a Biotechnologii | Matejicek, A., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic) | Kaplan, J., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic)
Elderberry is still not a certified fruit in the Czech Republic, however, elderberry production orchards have been started. Elderberry branches left after cutting are a valuable source of a pharmaceutical matter ? flavonoid rutin, which could be used for food supplement or pharmaceutical production. Therefore, this paper is focused on determining rutin and its aglycone quercetin in waste branches and leaves of selected elderberry cultivars (Albida, Bohatka, Dana, Haschberg, Koersoer and wild elderberry). Flavonoids were extracted by a new technique called PSE (pressurized solvent extraction), and determined by high performance liquid chromatography. The highest amount of rutin was determined in wild elderberry leaves - 5.58 mg/g, among the cultivars it was 2.28 mg/g in Haschberg cultivar leaves. Least rutin was contained in Koersoer cultivar leaves - 0.13 mg/g. Analyzing dry grinded branches, the highest rutin content was found in colourless Albida cultivar - 2.33 mg/g, the lowest content 0.07 mg/g was determined in wild elderberry branches. Content of quercetin was insignificant both in leaves and branches of all cultivars except for the wild elderberry (0.24 mg/g). From this we may conclude, that most of the valuable matters in elderberry occurs in form of rutin, which can be easily isolated by the PSE technique. Our assumption that waste elderberry branches are valuable and usable source of pharmaceutically significant matter has been proved.
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