Effect of natural substances as a potential substitute for antibiotics in boar semen extender on semen survival time
2012
Frydrychova, S., Institute of Animal Science, Prague (Czech Republic) | Lustykova, A., Institute of Animal Science, Prague (Czech Republic) | Vaclavkova, E., Institute of Animal Science, Prague (Czech Republic) | Lipensky, J., Institute of Animal Science, Prague (Czech Republic) | Rozkot M., Institute of Animal Science, Prague (Czech Republic) | Opletal, L., Charles University in Prague (Czech Republic). Faculty of Pharmacy
The objective of the experiment was to determine the effect of natural substances as a potential substitute for antibiotics in the boar extender on the semen survival time. Thirteen natural substances were tested. Natural substances were dissolved in 4% DMSO with a minimum bactericidal effective concentration of between 300 and 4,800 microg/ml. The control sample was diluted with a semen dilution ratio of 1:1 in a BTS extender without antibiotics. Sperm motility was evaluated at 0h, 1h and 24h after semen dilution. The best sperm motility was only recorded at thymol 25 % and carvacrol 40 % after 24h storage time. Other tested natural substances had sperm motility between 0 and 5 %. The sperm motility was significantly decreased over the storage time for all the tested natural substances (P less than 0.01). Results of the present study indicate a negative effect of the concentrations of tested natural substances on boar semen survival time. At present, practical utilization of the natural substances we tested as a potential substitute for antibiotics in boar semen extenders is not possible owing to its reduced sperm motility.
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