Structural changes of region 1-16 of the Alzheimer disease amyloid β-peptide upon zinc binding and in vitro aging
2006
Zirah , Séverine (Muséum National d’Histoire NaturelleCentre National de la Recherche Scientifique, ParisParis(France). Département Régulations, Développement et Diversité Moléculaire, Laboratoire de Chimie et Biochimie des Substances NaturellesUMR 5154) | Kozin , Serguey A. (Muséum National d’Histoire NaturelleCentre National de la Recherche ScientifiqueRussian Academy of Medical Sciences, ParisParisMoscow(France). Département Régulations, Développement et Diversité Moléculaire, Laboratoire de Chimie et Biochimie des Substances NaturellesUMR 5154Institute of Biomedical Chemistry, Proteomics Department) | Mazur , Alexey K. (Centre National de la Recherche Scientifique, Paris(France). UPR 9080, Institut de Biologie Physico-Chimique) | Blond , Alain (Muséum National d’Histoire NaturelleCentre National de la Recherche Scientifique, ParisParis(France). Département Régulations, Développement et Diversité Moléculaire, Laboratoire de Chimie et Biochimie des Substances NaturellesUMR 5154) | Cheminant , Michel (Muséum National d’Histoire NaturelleCentre National de la Recherche Scientifique, ParisParis(France). Département Régulations, Développement et Diversité Moléculaire, Laboratoire de Chimie et Biochimie des Substances NaturellesUMR 5154) | Ségalas-Milazzo , Isabelle (Université de RouenCentre National de la Recherche Scientifique, Mont-Saint-AignanMont-Saint-Aignan(France). Laboratoire de Résonance Magnétique Nucléaire (RMN), Institut de Recherche en Chimie Organique Fine (IRCOF)UMR 6014, IFRMP 23 Institut Fédératif de Recherches Multidisciplinaires sur les Peptides) | Debey , Marie Pascale (INRA (France). UMR 1198 Biologie du Développement et Reproduction) | Rebuffat , Sylvie (Muséum National d’Histoire NaturelleCentre National de la Recherche Scientifique, ParisParis(France). Département Régulations, Développement et Diversité Moléculaire, Laboratoire de Chimie et Biochimie des Substances NaturellesUMR 5154)
Amyloid deposits within the cerebral tissue constitute a characteristic lesion associated with Alzheimer disease. They mainly consist of the amyloid peptide Aβ and display an abnormal content in Zn2+ ions, together with many truncated, isomerized, and racemized forms of Aβ. The region 1-16 of Aβ can be considered the minimal zinc-binding domain and contains two aspartates subject to protein aging. The influence of zinc binding and protein aging related modifications on the conformation of this region of Aβ is of importance given the potentiality of this domain to constitute a therapeutic target, especially for immunization approaches. In this study, we determined from NMR data the solution structure of the Aβ-(1-16)-Zn2+ complex in aqueous solution at pH 6.5. The residues His6, His13, and His14 and the Glu11 carboxylate were identified as ligands that tetrahedrally coordinate the Zn(II) cation. In vitro aging experiments on Aβ-(1-16) led to the formation of truncated and isomerized species. The major isomer generated, Aβ-(1-16)-l-iso-Asp7, displayed a local conformational change in the His6-Ser8 region but kept a zinc binding propensity via a coordination mode involving l-iso-Asp7. These results are discussed here with regard to Aβ fibrillogenesis and the potentiality of the region 1-16 of Aβ to be used as a therapeutic target.
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